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A. It is possible it could be play behavior but without seeing it in person, hard to say. Is the male kitten neutered? You may want to consider doing so. Also, try re-introducing the kittens slowly by creating a safe space for the new kitten behind a closed door in a room. Keep her there for at least a week so she is protected but your male is still able to smell her. After a week or 2, you can then graduate to using a baby gate so they can then not only smell each other but safely see each other as well. If that is going okay, after another few days you can bring the gate down. Also, be sure to have feeding bowls in separate locations and at least 2 litter boxes.
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